DNA barcodes for insect pest identification: a test case with tussock moths (Lepidoptera: Lymantriidae) | Semantic Scholar (2024)

Results demonstrate that COI DNA barcodes are useful for Bradysia species identification and reveal a complex of three species within the B. ocellaris group, which may consist of at least three species.

Both PCR-RFLPs and DNA barcoding could provide quick and accurate tools for identification of aphid species within Aphididae subsequently aiding in effective pest management programmes and enhance plant quarantine systems.

This study demonstrates the efficacy of DNA barcodes for diagnosing species of Lymantria and reinforces the view that the approach is an under-utilized resource with substantial potential for biosecurity and surveillance.

The integration of DNA barcoding into routine biosurveillance and forest insect surveys to prevent such delay in recognition of non-indigenous species—in this case, 34 years.

It is indicated that DNA barcoding has the potential to be an efficient and accurate supplement to morphological methods for the identification of invasive Spodoptera pests in North America.

DNA of tussock moth and fruit fly specimens intercepted at the New Zealand border over the last decade were reanalysed using the cox1 sequence barcode approach, highlighting several advantages of DNA barcodes, especially their adaptability and predictive value.

Because of their importance as pests and disease vectors impacting global agriculture and forestry, DNA barcode results on these arthropods have significant implications for quarantine detection, regulation, and management.

The present chapter deals with the use of barcode in the identification of insects belonging to different orders and families, using the neighbour-joining approach with the bootstrapping method and the Kimura-2 parameter to obtain a clear phylogenetic signal.

...

...

A molecular method is described, using simple restriction patterns of ribosomal DNA (rDNA) as diagnostic markers, that could be useful as a diagnostic technique and discuss directions for further development.

DNA of tussock moth and fruit fly specimens intercepted at the New Zealand border over the last decade were reanalysed using the cox1 sequence barcode approach, highlighting several advantages of DNA barcodes, especially their adaptability and predictive value.

A phylogenetically focussed approach is employed in the development of a diagnostic key to distinguish a range of leafroller species using DNA sequence data derived from a 2.3 kb region of the mitochondrial genome containing the genes cytochrome oxidase I and II.

It is concluded that DNA barcoding can provide a powerful tool for mayfly species identification and should be considered as a priority for further research.

Molecular identification has to focus not only on the species of complexes, but also on related species if they hamper the morphological identification of the ‘sensu lato species’.

A database of mitochondrial DNA sequence data is developed that makes it possible to identify all sarcophagid species likely to be found feeding on a human corpse at an urban location in Canada or the USA.

It is concluded that DNA barcoding is a powerful tool for identifying species of Collembola and should regularly be useful as a complement to traditional, morphological taxonomy.

The results add to the evidence that cryptic species are prevalent in tropical regions, a critical issue in efforts to document global species richness, and illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity.

A protocol for the nondestructive extraction of DNA from terrestrial arthropods is described, using as examples members of the orders Acarina, Araneae, Coleoptera, Diptera, and Hymenoptera chosen to represent the ranges in size, overall sclerotization, and delicacy of key morphological characters in the group.

...

...